作者: cumo (海帶) 站內: ColdBaimotoR
標題: Re: [生物] PCR之歌
時間: Fri Jan 18 02:53:32 2008

那我解釋一下,懂了的人就不用往下看了 orz

呂文司機拿了一件被滴到洨的衣服,說柯林頓跟她玩咬東西的遊戲
這幾滴乾掉的洨有DNA,但是少到靠背,很難分析出是誰的

但是利用PCR技術就可以將一段DNA複製為原來的一百億至一千億倍。

只要一滴洨

> The PCR Song
>  There was a time when to amplify DNA,
曾經需要放大 DNA
>  You had to grow tons and tons of tiny cells.
你必須要養很多很多的細菌(來複製DNA)
>  Then along came a guy named Dr. Kary Mullis,
直到一個叫做Dr. Kary Mullis的人突然出現
>  Said you can amplify in vitro just as well.
說你也可以在體外放大 DNA 喔 >.+
>  Just mix your template with a buffer and some primers,
只要你將你的模板DNA跟緩衝液還有引子混合
>  Nucleotides and polymerases, too.
還有核甘酸跟酵素喔~
>  Denaturing, annealing, and extending.
解開雙股螺旋~ 讓引子與模板DNA結合~ 伸長DNA~
>  Well it’s amazing what heating and cooling and heating will do.
熱一下 冷一下 熱一下 就完成了真是太神奇了~
>  PCR, when you need to detect mutations.
PCR~ 當你需要找出突變處時 ~
>  PCR, when you need to recombine.
PCR~ 當你需要基因重組時 ~
>  PCR, when you need to find out who the daddy is.
PCR~ 當你需要找你老爸時 ~
>  PCR, when you need to solve a crime.  (repeat chorus
PCR~ 當你需要找到犯人時 ~
> http://bio-rad.cnpg.com/lsca/videos/ScientistsForBetterPCR/
bio-rad是一家儀器公司

--
  Origin:  玩車人的天地 ˙東吳機研站  scumotor.com.tw 
  Author: cumo 59-105-90-28.adsl.dynamic.seed.net.tw 發表miltonian :這是原曲WE ARE THE WORLD                             08/01/18
→ miltonian :http://www.youtube.com/watch?v=WmxT21uFRwM           08/01/18
→ davidch :there was a time翻成 曾經 比較好吧?                    08/01/18
謝謝 我英文很濫 XD
  Modify: 08/01/18 18:51:40