作者: cumo (海帶) 站內: ColdBaimotoR
標題: Re: [生物] PCR之歌
時間: Fri Jan 18 02:53:32 2008
那我解釋一下,懂了的人就不用往下看了 orz
呂文司機拿了一件被滴到洨的衣服,說柯林頓跟她玩咬東西的遊戲
這幾滴乾掉的洨有DNA,但是少到靠背,很難分析出是誰的
但是利用PCR技術就可以將一段DNA複製為原來的一百億至一千億倍。
只要一滴洨
> The PCR Song
> There was a time when to amplify DNA,
曾經需要放大 DNA
> You had to grow tons and tons of tiny cells.
你必須要養很多很多的細菌(來複製DNA)
> Then along came a guy named Dr. Kary Mullis,
直到一個叫做Dr. Kary Mullis的人突然出現
> Said you can amplify in vitro just as well.
說你也可以在體外放大 DNA 喔 >.+
> Just mix your template with a buffer and some primers,
只要你將你的模板DNA跟緩衝液還有引子混合
> Nucleotides and polymerases, too.
還有核甘酸跟酵素喔~
> Denaturing, annealing, and extending.
解開雙股螺旋~ 讓引子與模板DNA結合~ 伸長DNA~
> Well it’s amazing what heating and cooling and heating will do.
熱一下 冷一下 熱一下 就完成了真是太神奇了~
> PCR, when you need to detect mutations.
PCR~ 當你需要找出突變處時 ~
> PCR, when you need to recombine.
PCR~ 當你需要基因重組時 ~
> PCR, when you need to find out who the daddy is.
PCR~ 當你需要找你老爸時 ~
> PCR, when you need to solve a crime. (repeat chorus
PCR~ 當你需要找到犯人時 ~
> http://bio-rad.cnpg.com/lsca/videos/ScientistsForBetterPCR/
bio-rad是一家儀器公司
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╭┼ Origin: 玩車人的天地 ˙東吳機研站 scumotor.com.tw ┼╯ Author: cumo從 59-105-90-28.adsl.dynamic.seed.net.tw 發表
→ miltonian推:這是原曲WE ARE THE WORLD 08/01/18
→ miltonian推:http://www.youtube.com/watch?v=WmxT21uFRwM 08/01/18
→ davidch推:there was a time翻成 曾經 比較好吧? 08/01/18
謝謝 我英文很濫 XD
╰╮Modify: 08/01/18 18:51:40